Uni Sitotoksisitas Ekstrak Air Asam Jawa 5% terhadap Cell Line BHK-21
نویسندگان
چکیده
منابع مشابه
Site specific integration of FLP recombinase in BHK-21 cell line.
A binary system for gene activation and site specific integration based on conditional recombination of transfected sequences mediated by FLP recombinase from yeast was implemented in mammalian cells. In several cell lines, FLP rapidly and precisely recombined copies of its specific target sequences to activate an otherwise silent beta-galactosidase reporter gene. Clones of marked cells were ge...
متن کاملPreservation and Maintenance of Bhk-21 Cell Line at Different Temperature Conditions
The present work was undertaken with an aim to standardize the preservation of BHK-21 cell lines at different temperature conditions viz., at 4 C , -20 C and in LN2 using appropriate freezing medium. The cell line after preservation was revived at 10 days and 40 days intervals. We observed that the cells maintained at 4 C and LN2 at -196 C were having the highest revival rates and within 24-36 ...
متن کاملContact of Entamoeba histolytica with baby hamster kidney-21 (BHK-21) cell line on cysteine proteinase activity.
BACKGROUND & OBJECTIVES Entamoeba histolytica, the causative agent of amoebiasis and amoebic liver abscess, lyses host cells by direct contact using surface lectins and releases cysteine proteinase (CP). Virulence of E. histolytica is directly related to activity of its CP. The relationship of CP activity and cytotoxicity has not been established. The present study was carried out to explore th...
متن کاملLipids of rabies virus and BHK-21 cell membranes.
The lipid composition of highly purified Flury strain of rabies virus (HEP) propagated in BHK-21 cells in a chemically defined medium was observed to be 6.7% neutral lipids, 15.8% phospholipids, and 1.5% glycolipids. In the virion, phosphatidylethanolamine, phosphatidylcholine, and sphingomyelin were the most abundant phospholipids, accounting for 90% of the total, and the molar ratio of choles...
متن کاملProduction of Rabies Vaccine Using BHK-21 with Roller Bottle Cell Culture Technique
Compared to other methods of vaccine production, (e.g. culture flask and bioreactor system), the roller culture method for producing rabies vaccine is simpler, and the equipment easier to install and maintain, and also in the event of contamination of one roller, the entire batch need not be discarded. This system provides a much larger surface area than the culture flask, and also ensures a mu...
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ژورنال
عنوان ژورنال: Journal of Dentistry Indonesia
سال: 2008
ISSN: 2355-4800,1693-9697
DOI: 10.14693/jdi.v14i1.787